%PDF-1.3
%
2 0 obj
<>
endobj
17 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 53/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T0 q-Br44C@B 9 s
(P
endstream
endobj
7 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 55/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T0P02S04S @$P5 )1
endstream
endobj
22 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 55/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T0P02S04S @$P5 )1
endstream
endobj
11 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 53/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T0 q-Br44C@B 9 s
(P
endstream
endobj
1 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 53/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T0 q-Br44C@B 9 s
(P
endstream
endobj
9 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 54/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T R
Ɩf
!\!Y\ !p )P
endstream
endobj
15 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 54/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T R
Ɩf
!\!Y\ !p )P
endstream
endobj
5 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 53/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T0 q-Br44C@B 9 s
(P
endstream
endobj
8 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 53/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T0 q-Br44C@B 9 s
(P
endstream
endobj
20 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 53/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T0 q-Br44C@B 9 s
(P
endstream
endobj
19 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 55/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T0P02S04S @$P5 )1
endstream
endobj
12 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 54/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T R
Ɩf
!\!Y\ !p )P
endstream
endobj
16 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 55/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T0P02S04S @$P5 )1
endstream
endobj
10 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 55/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T0P02S04S @$P5 )1
endstream
endobj
18 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 54/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T R
Ɩf
!\!Y\ !p )P
endstream
endobj
14 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 53/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T0 q-Br44C@B 9 s
(P
endstream
endobj
6 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 54/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T R
Ɩf
!\!Y\ !p )P
endstream
endobj
3 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 54/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T R
Ɩf
!\!Y\ !p )P
endstream
endobj
13 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 55/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T0P02S04S @$P5 )1
endstream
endobj
21 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 54/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T R
Ɩf
!\!Y\ !p )P
endstream
endobj
4 0 obj
<>>>/Subtype/Form/BBox[0 0 612 792]/Matrix [1 0 0 1 0 0]/Length 55/FormType 1/Filter/FlateDecode>>stream
xs
w3T04PI2P0T5T0P02S04S @$P5 )1
endstream
endobj
23 0 obj
<>stream
2012-05-30T10:50:47+05:30
Arbortext Advanced Print Publisher 9.0.114/W
2012-06-28T22:50:03-07:00
Acrobat Distiller 8.1.0 (Windows)
application/pdf
untitled
uuid:e0b2d985-8ec5-4570-bc15-7389126e4129
uuid:b8d0a2d8-67ce-4e35-b766-7d025f275616
endstream
endobj
24 0 obj
<>stream
x+ |
endstream
endobj
25 0 obj
<>stream
xS**T0T0 BiUD* 8
endstream
endobj
26 0 obj
<>stream
x+ |
endstream
endobj
27 0 obj
<>stream
xS**T0T0 Bih]9D. qaj
endstream
endobj
28 0 obj
<>stream
x+ |
endstream
endobj
29 0 obj
<>stream
xS**T0T0 Bih] D. pa
endstream
endobj
30 0 obj
<>stream
x+ |
endstream
endobj
31 0 obj
<>stream
xS**T0T0 BiU !D. _e1
endstream
endobj
32 0 obj
<>stream
x+ |
endstream
endobj
33 0 obj
<>stream
xS**T0T0 Bih]D. r^j
endstream
endobj
34 0 obj
<>stream
x+ |
endstream
endobj
35 0 obj
<>stream
xS**T0T0 BiU)D* 9V
endstream
endobj
36 0 obj
<>stream
x+ |
endstream
endobj
37 0 obj
<>stream
xS**T0T0 BiUD* :
endstream
endobj
38 0 obj
<>/ProcSet [/PDF /Text /ImageB /ImageC /ImageI]/XObject<>/Font<>>>/Thumb 52 0 R/MediaBox[0 0 612 792]/Rotate 0>>
endobj
53 0 obj
<>/ProcSet[/PDF/Text/ImageB]/XObject<>/Font<>>>
endobj
40 0 obj
<>stream
/GS1 gs
BT
/F4 1 Tf
8.9663 0 0 8.9663 62.022 725.7825 Tm
0 0 0 1 k
0 Tc
0 Tw
[(kocyte)-409.6(NADPH)-402.5(oxidase,)-407.1(found)-409.3(in)-405.3(professional)-406.3(phagocytes,)]TJ
0 -1.2203 TD
[(catalyzes)-342.6(the)-345.2(production)-341.1(of)-344.7(O)]TJ
6.7246 0 0 5.9768 180.6236 713.3101 Tm
(2)Tj
/F9 1 Tf
.4974 .9201 TD
()Tj
/F10 1 Tf
.666 0 TD
(-)Tj
/F4 1 Tf
8.9663 0 0 8.9663 196.3275 714.8408 Tm
[(by)-344(the)-338.9(one-electron)-351.6(reduc-)]TJ
-14.979 -1.2266 TD
[(tion)-212.5(of)-211.9(oxygen,)-216(using)-216.3(NADPH)-212.8(as)-209.5(the)-218.7(electron)-212.3(donor)-215(\(4,)-211.6(5\).)-211.6(It)-211.2(is)]TJ
0 -1.2203 TD
[(widely)-268.9(reported)-268(that)-269.4(patients)-269.2(with)-268.3(type)-265.4(II)-269.8(DM)-265.8(\(T2DM\))-269.2(suffer)]TJ
T*
[(from)-380.9(systemic)-387.2(oxidative)-382.3(stress)-384.4(\(16\).)-382.3(However,)-387.9(the)-383.1(ability)-379.8(of)]TJ
0 -1.2266 TD
[(peripheral)-380.6(blood)-370.6(monocytes)-378.6(of)-370(T2DM)-375.8(to)-376.3(mount)-372.3(respiratory)]TJ
0 -1.2203 TD
[(burst)-305.4(response)-307.8(in)-310.4(response)-307.8(to)-306.7(appropriate)-308.3(pathogenic)-308.9(stimu-)]TJ
T*
[(lus)-568.9(is)-573.9(known)-565.7(to)-572.3(be)-573.4(compromised,)-569.1(increasing)-575.4(the)-566.5(risk)-572.7(of)]TJ
0 -1.2266 TD
[(infection-related)-289.8(complications)-283.9(in)-285.1(diabetics)-280.5(\(14\).)]TJ
/F6 1 Tf
1.1128 -1.2203 TD
[(Carica)-231.7(papaya)-230.3(Linn)]TJ
/F4 1 Tf
8.0301 0 TD
[(is)-226.1(widely)-231(recognized)-231.4(as)-228.4(a)-233.5(medicinal)-228.2(fruit)]TJ
-9.1429 -1.2203 TD
[(\(2\).)-483.9(Fermented)-489.8(papaya)-479.9(preparation)-487.4(\(FPP\),)-485.2(a)-486.4(granular)-481(sub-)]TJ
T*
[(stance,)-297.6(is)-295.7(available)-292.4(over)-295(the)-294.6(counter)-298.7(\(32\).)-293.7(FPP)-298(possesses)-295(anti-)]TJ
0 -1.2266 TD
[(oxidant)-299.4(properties)-309.2(\(3,)-300.1(11,)-299.1(22,)-299.1(35\))-296.7(that)-301(provide)-302.9(benet)-301.9(against)]TJ
0 -1.2203 TD
[(age-related)-333.8(complications)-334.5(\(27\).)-331.7(FPP)-329.6(is)-327.3(also)-329.6(known)-331.7(to)-332(protect)]TJ
T*
[(red)-401.9(blood)-402.2(cells)-399.6(against)-404.2(oxidative)-401.3(damage)-402.3(\(27,)-400.8(28\))-397.8(and)-404.2(help)]TJ
0 -1.2266 TD
[(against)-303(severe)-299.6(forms)-306.3(of)-300.4(thalassemia)-300.4(\(1\).)-306.9(Several)-299.9(independent)]TJ
0 -1.2203 TD
[(observations)-349.6(convergently)-351.7(point)-346.6(toward)-349.5(the)-345.2(hypothesis)-350.6(that)]TJ
T*
[(treatment)-476.6(with)-470.6(papaya)-473.6(preparations)-476.1(may)-475.7(facilitate)-471.8(wound)]TJ
0 -1.2266 TD
[(healing)-498.2(responses)-498.8(\(2,)-496.1(17,)-495.1(21,)-495.1(30,)-495.1(33,)-495.1(34\).)-495.6(Chronic)-493.8(wounds)]TJ
0 -1.2203 TD
[(represent)-462.9(a)-461.1(major)-460.7(public)-462.4(health)-459.1(problem)-459.2(in)-462.2(diabetics.)-460.5(Our)]TJ
T*
[(previous)-302.5(studies)-301.7(have)-307(demonstrated)-302.8(that)-301(wound-site)-302.4(macro-)]TJ
T*
[(phages)-338.7(of)-338.4(diabetics)-337.4(are)-335.2(compromised)-338.6(in)-335.7(their)-336.8(ability)-335.6(to)-338.4(sup-)]TJ
0 -1.2266 TD
[(port)-384.7(wound)-385.2(healing)-384.4(\(23\).)-388.6(Recently,)-384.8(our)-384.2(laboratory)-385.5(reported)]TJ
0 -1.2203 TD
[(rst)-302.7(evidence)-306.8(demonstrating)-311.3(that)-307.3(FPP)-304.3(may)-298.7(improve)-303.7(diabetic)]TJ
T*
[(wound)-334.6(outcomes)-340.5(by)-331.3(specically)-341.1(inuencing)-335(the)-338.9(response)-339.4(of)]TJ
0 -1.2266 TD
[(wound-site)-327.7(macrophages)-325.9(and)-322(the)-326.2(subsequent)-327.7(angiogenic)-321.3(re-)]TJ
0 -1.2203 TD
[(sponse)-319.9(\(15\).)-325.4(FPP)-317(has)-320.4(a)-315.7(long)-317.6(track-record)-327.4(of)-319.4(safe)-317.3(human)-321.4(con-)]TJ
T*
[(sumption)-378.5(\(26,)-375.5(28\).)-369.1(The)-379.3(objectives)-377.7(of)-370(the)-376.8(current)-378.8(study)-377.3(were)]TJ
0 -1.2266 TD
[(twofold:)-453.8(to)-452.2(determine)-451(whether)-452(FPP)-456.1(is)-447.4(able)-450(to)-452.2(improve)-449.2(in-)]TJ
0 -1.2203 TD
[(ducible)-710.5(respiratory)-710.9(burst)-710.1(outcomes)-713.6(in)-708.8(peripheral)-709.4(blood)]TJ
T*
[(mononuclear)-292.4(cells)-292.1(\(PBMC\))-295.1(of)-287.8(diabetic)-298.2(patients,)-291.1(and)-290.4(to)-294.1(inves-)]TJ
0 -1.2266 TD
[(tigate)-400.3(the)-395.8(underlying)-400.7(mechanisms.)-401.7(The)-398.3(overall)-396.4(goal)-395.9(was)-400.8(to)]TJ
0 -1.2203 TD
[(develop)-283.1(data)-280.7(toward)-280(future)-288.8(phase-II)-282.1(clinical)-281.2(studies.)]TJ
/F3 1 Tf
0 -3.054 TD
(Results)Tj
/F4 1 Tf
1.1128 -1.8336 TD
[(Phorbol)-227.6(12-myristate)-235(13-acetate)-226.2(\(PMA\))-230.1(stimulated)-232(a)-227.1(potent)]TJ
-1.1128 -1.2203 TD
[(induction)-273.6(of)-268.8(superoxide)-275.2(anion)-271.8(production)-271.5(from)-273.4(PBMCs.)-266.8(This)]TJ
0 -1.2266 TD
[(response)-257.2(was)-249(severely)-257(blunted)-250(in)-253.5(PBMC)-249.9(isolated)-254.3(from)-254.4(T2DM)]TJ
0 -1.2203 TD
[(patients)-509.4(compared)-503.6(to)-509.1(results)-508.1(from)-507.3(normoglycemic)-506(control)]TJ
T*
[(human)-441.5(subjects.)-439.6(Intriguingly,)-442.9(the)-440(compromise)-443(in)-443.2(inducible)]TJ
0 -1.2266 TD
[(respiratory)-394.7(burst)-400.3(activity)-391.3(in)-392.6(T2DM)-394.7(PBMC)-395.3(was)-394.5(corrected)-398(in)]TJ
0 -1.2203 TD
[(response)-402.7(to)-395.3(exposure)-395.2(to)-395.3(FPP)-399.1(\(Fig.)-395.1(1\).)-394.9(FPP)-392.8(induces)-397.5(ROS)-397.8(ex-)]TJ
T*
[(pression)-412.6(in)-411.6(cells)-412.2(isolated)-406.1(from)-412.5(normoglycemic)-411.2(subjects;)-414.3(the)]TJ
T*
[(extent)-278.2(of)-268.8(induction,)-276.6(however,)-271.2(is)-270.4(less)-271.6(\(20%)]TJ
/F6 1 Tf
18.3997 0 TD
(vs.)Tj
/F4 1 Tf
1.4037 0 TD
[(230%)-272(in)-272.5(T2DM\))]TJ
-19.8034 -1.2266 TD
[(compared)-453(to)-452.2(cells)-450.2(from)-450.4(T2DM)-451.6(\(data)-451.9(not)-449.2(shown\).)-458.5(NADPH)]TJ
0 -1.2203 TD
[(oxidase)-233.5(represents)-230.2(the)-231.4(primary)-229.1(enzyme)-232.3(that)-231.5(is)-232.5(responsible)-230.8(for)]TJ
T*
[(PMA-inducible)-491.9(respiratory)-483.3(burst)-488.8(production)-486.5(in)-487.5(PBMC.)-480.5(To)]TJ
0 -1.2267 TD
[(investigate)-346(the)-345.2(underlying)-343.8(mechanisms)-341.9(of)-344.7(FPP)-342.2(action)-337.7(on)-341.3(re-)]TJ
0 -1.2203 TD
[(spiratory)-267.7(burst,)-270.4(isolated)-266.9(PBMCs)-263.9(were)-267.7(cultured)-268.8(in)-266.2(presence)-271.3(of)]TJ
T*
[(FPP)-418.1(for)-414.9(24)-169.7(h.)-417.3(Expression)-417.8(of)-420.6(the)-414.7(major)-416.5(subunits)-421.8(of)-420.6(NADPH)]TJ
0 -1.2266 TD
[(oxidase)-258.8(complex)-259.5(was)-261.7(determined)-257.9(using)-260.6(Western)-257.6(blot)-264.1(\(Fig.)-256(2\).)]TJ
0 -1.2203 TD
[(Interestingly,)-352.9(the)-345.2(expression)-351.4(of)-344.7(Rac2)-344(was)-343.9(signicantly)-352.3(upre-)]TJ
T*
[(gulated)-435.8(after)-431(FPP)-430.8(supplementation)-437.1(\(Fig.)-433.1(3A\).)-434.3(FPP)-430.8(contains)]TJ
0 -1.2266 TD
[(large)-355.8(amounts)-360.5(\()]TJ
/F11 1 Tf
7.0437 0 TD
(*)Tj
/F4 1 Tf
.999 0 TD
[(90%\))-355.2(of)-357.3(carbohydrates)-360.8(\(29\).)-350.6(We)-359.3(have)-357.6(pre-)]TJ
-8.0427 -1.2203 TD
[(viously)-548.8(shown)-556.3(that)-547.6(matching)-549(amount)-555.2(of)-547(glucose)-550(supple-)]TJ
T*
[(mentation)-251.3(did)-243.5(not)-246.9(result)-247.5(in)-247.2(ROS)-246(production)-252.6(as)-241.1(observed)-246.7(after)]TJ
T*
[(FPP)-405.5(supplementation)-411.8(\(15\).)-401.2(To)-404.1(conrm)-405.1(these)-403.3(observation)-411.5(in)]TJ
0 -1.2266 TD
[(ROS)-277.6(production)-277.9(by)-280.7(human)-277.1(PBMC,)-271.8(glucose)-278.1(\(matching)-283.9(to)-275.1(that)]TJ
0 -1.2203 TD
[(present)-241(in)-240.9(FPP\))-241.6(treatment)-242.6(to)-243.5(the)-244(PBMCs)-238.6(was)-236.4(performed)-246.6(\(data)]TJ
T*
[(not)-202.6(shown\).)-199.2(Glucose)-202.9(treatment)-198.4(alone)-198.8(did)-199.2(not)-196.3(further)-203(stimulate)]TJ
0 -1.2267 TD
[(PMA-induced)-375.9(ROS)-378.8(production)-372.7(in)-373.6(PBMCs)-377.7(as)-373.9(observed)-373.1(after)]TJ
27.9978 33.0246 TD
[(FPP)-279(treatment,)-277.2(suggesting)-277.4(that)-275.7(FPP)-279(is)-276.7(specically)-271.6(effective)-282.9(in)]TJ
0 -1.2266 TD
[(inducing)-350.7(ROS)-353.5(production,)-350.3(which)-346.3(is)-352.6(independent)-350(of)-351(the)-345.2(glu-)]TJ
0 -1.2203 TD
[(cose)-452.2(present)-449.6(in)-443.2(this)-445(nutritional)-452.6(supplement.)-449.3(In)-448.1(addition)-442.8(to)]TJ
T*
[(upregulation)-306.8(of)-306.7(Rac2)-299.7(expression,)-310.1(FPP)-298(exposure)-306.6(also)-304.3(resulted)]TJ
T*
[(in)-323.1(increased)-326.7(phosphorylation)-325.5(of)-319.4(p47phox)-319.9(\(Fig.)-325.6(3B\).)-315.7(With)-322.6(the)]TJ
0 -1.2267 TD
[(goal)-433.9(to)-433.2(understand)-439.7(how)-431.4(FPP)-430.8(induces)-435.4(Rac2)-432.5(expression,)-436.6(the)]TJ
0 -1.2203 TD
[(effect)-367.2(on)-360.2(gene)-368(transcription)-368.2(was)-356.5(investigated.)-370.9(Expression)-360.9(of)]TJ
T*
[(Rac2)-400.9(mRNA)-395.2(was)-394.5(signicantly)-402.8(upregulated)-394.9(after)-399.4(FPP)-399.2(expo-)]TJ
0 -1.2266 TD
[(sure,)-271.7(suggesting)-264.8(that)-269.4(the)-263(Rac2)-268.1(transcriptional)-270.1(machinery)-264.6(was)]TJ
0 -1.2203 TD
[(sensitive)-298.6(to)-300.4(FPP.)-294.6(Maximal)-289.1(induction)-298.9(of)-294.1(Rac2)-293.4(mRNA)-300.4(was)-293.3(no-)]TJ
T*
[(ted)-186.7(after)-190.7(6)-163.9(h)-186.7(of)-186.6(treatment,)-188.7(indicating)-188.5(that)-187.2(the)-187.1(regulation)-190.3(by)-185.9(FPP)]TJ
0 -1.2267 TD
[(was)-249(rapid)-248.4(\(Fig.)-243.4(4A\).)-244.6(Next,)-252(to)-243.5(determine)-248.6(the)-250.4(minimum)-244.8(dose)-247.3(of)]TJ
0 -1.2203 TD
[(FPP)-253.7(required)-253.8(eliciting)-249.2(induction)-254.7(of)-249.8(Rac2)-255.5(mRNA)-249.8(expression,)-253.2(a)]TJ
T*
[(doseresponse)-421.8(study)-408.9(was)-413.4(performed.)-413.9(A)-406.1(dose)-411.7(of)-414.2(0.3)-166.3(mg/ml)]TJ
0 -1.2266 TD
[(was)-350.2(noted)-344.6(to)-344.7(be)-345.8(sufcient)-347.8(to)-344.7(signicantly)-352.3(induce)-345.6(Rac2)-344(tran-)]TJ
0 -1.2203 TD
[(scription,)-221.6(whereas)-215(the)-212.4(response)-219.3(was)-211.1(at)-215(a)-214.5(peak)-213.8(with)-211.3(1.5)-166.3(mg/ml)]TJ
T*
[(of)-281.4(FPP)-285.3(\(Fig.)-281.3(4B\).)]TJ
1.1128 -1.2203 TD
[(To)-347.2(test)-343.6(whether)-350.8(compromised)-344.9(inducible)-344.4(respiratory)-350.5(burst)]TJ
-1.1128 -1.2266 TD
[(function)-343.5(in)-329.4(PBMC)-338.4(from)-330.3(T2DM)-331.5(patients)-338.7(was)-337.6(associated)-333.1(with)]TJ
0 -1.2203 TD
[(lower)-394.2(Rac2)-381.9(expression,)-392.3(PBMC)-389(were)-387.8(obtained)-390(from)-387.2(patients)]TJ
T*
[(and)-284.1(subjected)-280.2(to)-281.4(RTPCR.)-281.5(Indeed,)-279.8(the)-282(expression)-281.9(of)-281.5(Rac2)-274.4(was)]TJ
0 -1.2266 TD
[(noted)-294.1(to)-294.1(be)-288.8(signicantly)-295.3(lower)-293.1(in)-291.5(PBMC)-287.9(of)-294.1(T2DM)-287.2(compared)]TJ
0 -1.2203 TD
[(to)-433.2(that)-427.5(in)-430.6(PBMC)-427(from)-431.5(normoglycemic)-430.1(control)-430.2(donors.)-434.2(The)]TJ
T*
[(lower)-229.8(expression)-231.3(of)-230.9(Rac2)-223.8(in)-228.2(PBMC)-230.9(of)-224.5(T2DM)-230.3(was)-223.8(signicantly)]TJ
0 -1.2266 TD
[(corrected)-284.2(in)-285.1(response)-282.5(to)-281.4(FPP)-285.3(treatment)]TJ
/F6 1 Tf
17.2932 0 TD
[(ex)-278.4(vivo)]TJ
/F4 1 Tf
3.1551 0 TD
[(\(Fig.)-281.3(5\).)]TJ
-19.3355 -1.2203 TD
[(The)-303.5(human)]TJ
/F6 1 Tf
5.3618 0 TD
(Rac2)Tj
/F4 1 Tf
2.3079 0 TD
[(gene)-298.5(promoter)-303.4(contains)-304.5(binding)-303.9(sites)-297.4(for)]TJ
-8.7825 -1.2203 TD
[(the)-256.7(transcription)-254.4(factors)-251.1(Sp1)-252.3(and)-252.5(AP-1.)-245.9(DNA)-255.7(binding)-247(activity)]TJ
T*
[(of)-433.2(AP-1)-432.7(and)-435.9(Sp1)-429.3(were)-432.1(therefore)-438.1(tested)-435(in)-430.6(response)-434.3(to)-433.2(FPP)]TJ
ET
q
223.145 0 0 196.724 321.165 535.521 cm
/Im1 Do
Q
BT
/F7 1 Tf
8.9663 0 0 8.9663 313.0582 518.74 Tm
[(FIG.)-545.5(1.)]TJ
/F5 1 Tf
4.3059 0 TD
[(Ex)-540(vivo)]TJ
/F7 1 Tf
4.1668 0 TD
[(supplementation)-544.1(of)-542.6(fermented)-544.1(papaya)]TJ
-8.4727 -1.1128 TD
[(preparation)-880.5(\(FPP\))-883.9(corrects)-876.2(type)-880(II)-880.3(diabetes)-877.3(mellitus)]TJ
0 -1.1065 TD
[(\(T2DM\)-mediated)-382.9(compromised)-374.9(respiratory)-379.9(burst)-373.2(produc-)]TJ
0 -1.1128 TD
[(tion)-868.3(by)-861(human)-868.6(peripheral)-863.5(blood)-862.6(mononuclear)-864.8(cells)]TJ
T*
(\(PBMC\).)Tj
/F4 1 Tf
4.1921 0 TD
[(Peripheral)-311.1(blood)-307.4(was)-306(collected)-308.8(from)-311.3(T2DM)-306.2(donors)]TJ
-4.1921 -1.1128 TD
[(with)-261.9(good)-265.9(glycemic)-258.5(control)-265.8(\(hemoglobin)-260.1(A1c)-265.8([HbA1c])]TJ
/F10 1 Tf
23.9449 0 TD
(<)Tj
/F4 1 Tf
.7145 0 TD
[(7\))-259.2(or)]TJ
-24.6594 -1.1065 TD
[(healthy)-254.9(normoglycemic)-246.8(\(NG\))-246.3(subjects)-253.3(\(CTL\).)-244(Freshly)-251.6(isolated)]TJ
0 -1.1128 TD
[(mononuclear)-387.2(leukocytes)-377.8(\(PBMC\))-383.6(were)-381.5(cultured)-376.3(in)-380(presence)]TJ
T*
[(or)-572.5(absence)-570.4(of)-572.3(FPP)-569.9(\(3)-164.4(mg/ml\))-569.3(for)-573(24)-163.4(h.)-569(Superoxide)-575.9(anion)]TJ
0 -1.1065 TD
[(production)-587.7(was)-584.2(measured)-585(after)-589.1(phorbol)-581.7(12-myristate)-589.1(13-)]TJ
0 -1.1128 TD
[(acetate)-688.2(\(PMA;)-681.5(1)]TJ
/F12 1 Tf
7.9226 0 TD
(l)Tj
/F4 1 Tf
.5501 0 TD
[(g/ml\))-679.2(activation)-683.4(for)-686.8(30)-163.4(min.)-677.6(Data)-683.8(are)]TJ
-8.4727 -1.1128 TD
[(expressed)-578.6(as)-576.2(percent)-580.4(change)-572.6(compared)-579.5(to)-572.3(corresponding)]TJ
T*
[(PMA-unstimulated)-270.3(cells.)-257.1(Data)-260.1(are)-259.3(mean)]TJ
/F10 1 Tf
17.6536 0 TD
()Tj
/F4 1 Tf
.7208 0 TD
[(standard)-257.5(deviation)]TJ
-18.3744 -1.1065 TD
(\(SD;)Tj
/F6 1 Tf
2.1245 0 TD
(n)Tj
/F10 1 Tf
.7208 0 TD
(=)Tj
/F4 1 Tf
.7145 0 TD
[(5\).)-262.1(*)]TJ
/F6 1 Tf
1.7198 0 TD
(p)Tj
/F10 1 Tf
.6639 0 TD
(<)Tj
/F4 1 Tf
.7208 0 TD
[(0.05)-254.4(compared)-257(to)-262.5(CTL.)]TJ
/F11 1 Tf
6.7246 0 0 5.9768 464.3715 393.2219 Tm
({)Tj
/F6 1 Tf
8.9663 0 0 8.9663 467.7164 389.2534 Tm
(p)Tj
/F10 1 Tf
.6702 0 TD
(<)Tj
/F4 1 Tf
.7145 0 TD
[(0.005)-260.2(compared)-257(to)]TJ
-18.6336 -1.1128 TD
[(nonsupplemented)-345.1(\(NS\))-334.5(T2DM)-337.8(group.)]TJ
/F3 1 Tf
-27.9978 41.3076 TD
(486)Tj
45.114 0 TD
[(DICKERSON)-338.9(ET)-338.7(AL.)]TJ
ET
endstream
endobj
42 0 obj
<>stream
Adobe d C
s !1AQa"q2B#R3b$r%C4Scs5D'6Tdt&
EFVU(eufv7GWgw8HXhx)9IYiy*:JZjz ? NlٳdO[Β/e7DJ5;8?1?/=mkVZޛ4P^Pm~GF+kq0>yZ[b/&ORG2+74PM5k!i6qu7 du-z66S@~ms"0rBo6lٳf͛6lٳf͛6lٳf͛6lٳf͛6l?ߚDѴ}{D
a:$$LjcQL No]pOoI3)|4r$hF"vlٳf͛6lٲ?Mɚžm-ގ-I#^+7KO?˚/<^ZYؘiP:^H9aJk\cͺYPmBPm p-F,HcE̾eX%q%xKH[('6u}.o4m;Fwt\2W̍'^Xoo_j˝@!DKPOy Qr6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lϿ4/0w
ή,'q},r#V .UYzc}rIG~&히DDEDQ@UQ l ͛6lٳf͛6lk֎kk
FHP+>aq*M=SKrH̴$Tc緷qM}P~`f%$%1ơTw
d}5P'F" 'ȗ_6nlٳf͛6lٳf͛6lٳf͛6lٳf͛6lٲ? (u &[97I? g͛6lٳf͛6lٳf͛6D* .Ksf͛6B5ίMUJu_ۿ$&:WƟNJt=sL4}WKatXGِ+
qf͛6lٳf͛6lٳf͛6lٳf͈_X_Oey6x#, +SVSH,NGi2rsV5bf͛6lٳf͛6lo26P4G̽ #.P8.bYv9U AoM<I#P=*%T2F؊dAR#2_"_{%f͛9?AgF͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6F3F/4ӵc7կeB7e;'϶y55/|)P B4/>S 1jv