Research

Effect of a quality-controlled fermented nutraceutical on skin aging markers: An antioxidant-control, double-blind study

Effect of a quality-controlled fermented nutraceutical on skin aging markers:
An antioxidant-control, double-blind study

EXPERIMENTAL AND THERAPEUTIC MEDICINE. 11(3):909-916, 2016

full paper download

The aim of the present study was to determine whether oral supplementation with a fermented papaya preparation (FPP treated group) or an antioxidant cocktail (antioxidant control group, composed of 10 mg trans resve¬ratrol, 60 μg selenium, 10 mg vitamin E and 50 mg vitamin C) was able to improve the skin antioxidant capacity and the expression of key skin genes, while promoting skin antiaging effects.
The study enrolled 60 healthy non smoker males and females aged 40 65 years, all of whom showed clinical signs of skin aging. The subjects were randomly divided into two matched groups, and were administered FPP or antioxidant treatment of a 4.5g sachet sublingually twice a day for 90 days in a double blind fashion.
The parameters investigated were: Skin surface, brown spots, skin evenness, skin mois¬turization, elasticity (face), redox balance, nitric oxide (NO) concentration, and the expression levels of key genes (outer forearm sample).
As compared with the baseline (day 0) and antioxidant control values, FPP treated subjects showed a significant improvement in skin moisturization (Fig.1), elasticity (Fig.2) and evenness (Fig.3). The two treatments improved the MDA and SOD skin concentrations, but only the FPP treated group showed a higher SOD level and a significant NO increase, along with significant upregulation of acquaporin 3(Fig.4 left) and downregulation of the potentially pro aging/carcinogenetic cyclophilin A (Fig.4 right) and CD147 genes (Fig.5 left). Progerin was unaffected in both treatment groups (Fig.5 right).
In conclusion, these findings suggest that orally administered FPP showed a consistent biological and gene regulatory improvement in the skin, as was also demonstrated in previous experimental and clinical trials testing other tissues, while common oral antioxidants had only a minor effect.


【Effects of FPP on Skin Aging Markers】

2016_skin_ExpTherMed_en_0.gif2016_skin_ExpTherMed_1.gif
Fig.1 Skin moisturization.
*P<0.05 vs. baseline value and §P<0.05 vs. antioxidant-control

2016_skin_ExpTherMed_2.gif
Fig.2 Skin elasticity parameter R6 (Uv/Ue).
*P<0.01 vs. baseline value and§P<0.05 vs. antioxidant-control

2016_skin_ExpTherMed_3.gif
Fig.3 Skin surface evenness, determined based on VAS score.
*P<0.05 vs. baseline value and §P<0.05 vs. antioxidant-control

2016_skin_ExpTherMed_4.gif
Fig.4 Skin gene expression of AQP-3 and CyPA.
*P<0.05 vs. baseline value and §P<0.05 vs. antioxidant-control

2016_skin_ExpTherMed_5.gif
Fig.5 Skin gene expression of CD147 and Progerin.
*P<0.05 vs. baseline value and §P<0.05 vs. antioxidant-control

List of the related papers

Year

Name of Papers